Multiple-laser microplate cytometers: adding a third dimension to high-content screening
نویسنده
چکیده
dimension to high-content screening The Acumen® eX3 microplate cytometer is the next generation of the Acumen Explorer® high-content screening system. It is equipped with multiple lasers, offering a wavelength range for excitation that greatly increases the variety of fluorescent reagents compatible with the system. The Acumen eX3 simplifies assay transfer by combining the powerful object recognition of charge-coupled device (CCD) imagers with the fast reads of bulk fluorescence readers.
منابع مشابه
Determination of cell colony formation in a high-content screening assay.
The use of cell colony formation assays for research and clinical applications to assess the functional integrity of cells after in vitro manipulations is extensive. Key areas include hematopoietic stem cell research, cell transformation studies, and predicting the response of tumors to chemotherapeutic agents. Traditionally, enumeration of colonies has involved laborious and subjective countin...
متن کاملStep-by-step guide to building an inexpensive 3D printed motorized positioning stage for automated high-content screening microscopy.
High-content screening microscopy relies on automation infrastructure that is typically proprietary, non-customizable, costly and requires a high level of skill to use and maintain. The increasing availability of rapid prototyping technology makes it possible to quickly engineer alternatives to conventional automation infrastructure that are low-cost and user-friendly. Here, we describe a 3D pr...
متن کاملMicrofabricated multiple field of view imaging flow cytometry.
The combination of microscopy and flow cytometry enables image based screening of large collections of cells. Despite the proposition more than thirty years ago, adding high resolution wide-field imaging to flow cytometers remains challenging. The velocity of cells in flow cytometry can surpass a meter per second, requiring either sub-microsecond exposure times or other sophisticated photodetec...
متن کاملRapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays
Expression profiling on a large scale, as is the case in drug discovery, is often accomplished through use of sophisticated solid-phase protein microarrays or multiplex bead technologies. While offering both high-throughput and high-content analysis, these platforms are often too cost prohibitive or technically challenging for many research settings. Capitalizing on the favorable attributes of ...
متن کاملQuantitative Beutler test for newborn mass screening of galactosemia using a fluorometric microplate reader.
BACKGROUND The Beutler enzyme spot test is an effective assay for newborn mass screening of galactosemia, but it is qualitative and relies on visual interpretation. We describe a quantitative, instrumental modification of the assay. METHODS We modified the macroscopic visual Beutler enzyme spot test by adding extraction of blood components from filter paper, deproteinization with acetone-meth...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006